Technical Notes

This page contains some thoughts and hints how to setup the technical work-up of native and transplant kidney biopsies. Although these recommendations were put together several year ago, they are generally still valid in 2020.

General Notes

Technical work-up of kidney biopsies – an introduction (M.J. Mihatsch, 2012).

General recommendations – in short (M.J. Mihatsch, 2012).

Immunohistochemistry & electron microscopy (M.J. Mihatsch, 2012).

Systematic biopsy evaluation (M.J. Mihatsch, 2012).

Example of clinical data sheets for native and transplant biopsies (M.J. Mihatsch, 2012).

Histology

All about light microscopy (M.J. Mihatsch, 2012).

Immunofluorescence

Freezing and storage of biopsies (M.J. Mihatsch, 2012).

Immunofluorescence using frozen sections (M.J. Mihatsch, 2012).

Antibodies for immunofluorescence on frozen sections (M.J. Mihatsch, 2012).

Fix-IF: Immunofluorescence on paraffin sections (M.J. Mihatsch, 2012).

Immunohistochemistry

Immunohistochemistry using paraffin sections (M.J. Mihatsch, 2012).

Antibodies for immunohistochemistry on paraffin sections (M.J. Mihatsch, 2012).

C4d and SV40 staining by immunohistochemistry (M.J. Mihatsch, 2012).

Fibronectin immunohistochemistry (M.J. Mihatsch, 2012).

Amyloid typing by immunohistochemistry (M.J. Mihatsch, 2012).

Electron Microscopy

Standard procedure for electron microscopy (M.J. Mihatsch, 2012).

Immunoelectron microscopy (M.J. Mihatsch, 2012).

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